Peptide reconstitution: a research guide
Bacteriostatic water, syringe selection, dose math, storage. The reference we wish existed when we started.
published · · 1mo ago
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The setup
Lyophilized peptides ship as dry powder in vials. Reconstitution is the process of dissolving the powder in a sterile diluent — typically bacteriostatic water — to produce a solution that can be measured and dosed.
This guide covers the mechanics from a research-protocol perspective. None of it is human-use guidance.
Dose math
The relationship between mg of peptide, mL of bacteriostatic water, and dose-volume in IU on an insulin syringe is straightforward but easy to get wrong. The shortcut: (mg of peptide ÷ mL of bacteriostatic water) gives mg/mL concentration. From there, multiply by the dose volume in mL to get the dose in mg.
Most research uses 1 IU = 0.01 mL on a standard insulin syringe.
Storage
After reconstitution, peptides should be refrigerated (2–8°C) and protected from light. Most peptides are stable for 14–30 days at refrigerator temperature; longer storage requires freezing in single-dose aliquots to avoid freeze-thaw cycles.
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